Cst gfp antibody
WebThe GFP-mCherry-LC3 adenovirus (C3011, Beyotime Biotech, Shanghai, China) was used to track the LC3 protein. ... PVDF membranes were incubated with HRP-conjugated secondary antibody (#7074, CST) for 1 h. Following dropwise addition of ECL developer (P0018F, Beyotime Biotech, Shanghai, China), the immunoreactive protein bands were … WebApr 6, 2024 · I used the antibody from Cell Signaling Technology with 1:400 dilute and second antibody 1:400. ... Can Anti-GFP antibody used for labelling YFP as well? Question. 7 answers. May 7, 2024;
Cst gfp antibody
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WebPolyclonal Antibody for studying GFP jellyfish. Cited in 252 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, GFP Antibody (CST #2555) is ready to ship. A Protein Modification Resource. PhosphoSitePlus ® is an online systems … Each CST antibody is stability tested by exposure to room temperature and 37°C … WebMar 30, 2024 · GFP Antibody (B-2) is an IgG2a κ mouse monoclonal GFP antibody that detects GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA. GFP Antibody (B-2) is available as the non …
WebGFP 基因所产生的蛋白质，在蓝色波长范围的光线激发下会发出绿色萤光。GFP 标签可融合于蛋白质的 C 端或 N 端，GFP 抗体不仅可以检测 GFP 或其适当的突变体 EGFP 等，也可以检测其融合表达蛋白的表达、定位纯化，该系统已广泛应用于各种细胞类型，包括细菌 ... WebAntibodies that detect turboGFP can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunoprecipitation, ChIP and Flow Cytometry. …
WebSpecificity. Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On … WebApr 12, 2024 · Anti-FAP antibody was selected from a naïve scFv phage library. Then the antibody was converted into scFv-Fc format, transiently expressed by 293F cells and purified using protein A affinity chromatography. ... (CST), anti-CD4 (Abcam) and anti-CD8α (Abcam). Briefly, ... NIH 3T3 was transduced by pWPT-GFP-mFAP lentivirus and further …
WebGFP형광 확인하였는데 WB에 없네요.. GFP tagging된 DNA transcfection실험을 하고 있습니다. 그런데 분명 형광현미경으로 GFp가 발현하는 것을 보고 세포를 프랩했고 웨스턴을 했는데 웨스턴에서는 아무것도 안나오네요. 안티바디도 다른 회사것으로 바꾸어서 해 ...
WebFeb 12, 2024 · Western blot analysis of HeLa whole cell lysate (Lane 1) and GFP-transfected HeLa cell lysates (Lane 2). Samples were loaded at 20µg per lane onto a 15% SDS-polyacrylamide gel, transferred to a PVDF … shared prosperity funding cornwallWebOct 19, 2024 · Cell Signaling Technology. 3 Trask Lane. Danvers, MA 01923. [email protected]. www.cellsignal.com. 8776162355. headquarters: USA. Established … pool towel holder diyWeb66002-1-Ig targets GFP tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. … pool towel and swimsuit holder ideasWebMonoclonal Antibody for studying GFP jellyfish. Cited in 630 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). … pool towel cabinet outdoorWebOct 29, 2024 · Li Z et al detected GFP–LC3B and free GFP (from lysosomal cleavage) in the lysates of HeLa cells stably expressing GFP–LC3B with the Cell Signaling Technologies anti-GFP rabbit monoclonal antibody (a pd:1465972>2956) . Lundby A et al evaluated the GFP expression in A549 cells with the same anti-GFP antibody in Western blots . shared prosperity fund glasgowWebAntibodies that detect GFP can be used in several scientific applications, including Western Blot, ELISA, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Our … pool towel holder standWebThe GFP (D5.1) Rabbit mAb Antibody has been validated for the following applications: Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot. GFP (D5.1) XP® Rabbit mAb detects GFP, YFP, and CFP … shared prosperity fund technical guidance