Dynamic mass redistribution assay
WebAn optical dynamic mass redistribution assay reveals biased signaling of dualsteric GPCR activators. Anna Kebig Pharmacology and Toxicology Section, Institute of Pharmacy, ... An optical biosensor technique was applied to measure activation-induced dynamic mass redistribution (DMR) in CHO cells stably expressing the muscarinic receptor subtype ... WebAug 10, 2012 · Dynamic Mass Redistribution Assay Decodes Differentiation of a Neural Progenitor Stem Cell - Sadashiva Pai, Florence Verrier, Haiyan Sun, Haibei Hu, Ann M. Ferrie, Azita Eshraghi, Ye Fang, 2012 Skip to main content Intended for healthcare professionals 0Cart MENU Search Browse Resources Authors Librarians Editors Societies Advanced …
Dynamic mass redistribution assay
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WebOct 1, 2012 · Dynamic Mass Redistribution Assays The ReN cells were seeded in a freshly prepared laminin-coated biosensor microplate at 3000 cells per well at passages 2 to 10 … WebNov 1, 2024 · Introduction The label-free dynamic mass redistribution-based assay (DMR) is a powerful method for studying signalling pathways of G protein-coupled receptors (GPCRs). Herein we present the label-free DMR assay as a robust readout for pharmacological characterization of formyl peptide receptors (FPRs) in human neutrophils. Methods
WebFeb 17, 2024 · Here, we developed a novel assay for the human sweet taste receptor where endogenous G proteins and signaling pathways are recruited by the activated receptor. The effects of several sweet taste... WebJun 10, 2024 · Stimulation of the cells can lead to a dynamic mass redistribution (DMR) of cellular constituents and subsequently a change in refractive index. This leads to a shift of the resonant...
WebMay 1, 2024 · The DMR assay provides a robust and efficient platform to characterize pharmacological agents acting on adipocytes, which was used to further explore the potential activity of the bone-derived hormone osteocalcin. 2. Materials and methods 2.1. Isolation of primary preadipocytes WebDec 3, 2024 · Dynamic mass redistribution assay. Confluent cells were subcultured using trypsin/EDTA and used for experiments. Cells were seeded at a density of 20 000 cells/well in 30 μL into fibronectin‐coated Enspire™‐LC 384‐wells plates and cultured for 20 hours to form a confluent monolayer. On the day of the experiment, cells were manually ...
WebJan 10, 2024 · Dynamic mass redistribution as a means to measure and differentiate signaling via opioid and cannabinoid receptors Assay Drug Dev. Technol. , 9 ( 4 ) ( 2011 ) , pp. 362 - 372 CrossRef View Record in Scopus Google Scholar
WebPai, S., Verrier, F., Sun, H., Hu, H., Ferrie, A. M., Eshraghi, A., & Fang, Y. (2012). Dynamic Mass Redistribution Assay Decodes Differentiation of a Neural ... greater boston light showflight 意味 動詞WebDynamic Mass Redistribution Assays F11 cells were seeded in 384-well EnSpire-LFC plates (6057408; PerkinElmer) pretreated with laminin and poly- d -lysine. Cells were seeded at a density of 10 4 cells/well in 50 µL of culture medium and grown in a humidified atmosphere containing 5% carbon dioxide at 37 °C for 24 h. greater boston legal services numberWebOct 1, 2012 · Dynamic Mass Redistribution Assays The ReN cells were seeded in a freshly prepared laminin-coated biosensor microplate at 3000 cells per well at passages 2 to 10 suspended in 50 µL of growth factors containing medium and were cultured at 37 °C under 5% CO 2 for 2 days to reach ~90% confluency. greater boston legal services madeWebOct 20, 2011 · Label-free dynamic mass redistribution (DMR) is a cutting-edge assay technology that enables real-time detection of integrated cellular responses in living cells. … flight 翻译WebStem cells hold great potential in drug discovery and development. However, challenges remain to quantitatively measure the functions of stem cells and their differentiated products. Here, we applied fluorescent imaging, quantitative real-time PCR, and label-free dynamic mass redistribution (DMR) assays to characterize the differentiation process of … greater boston mediationWebDynamic mass redistribution : a novel physiological signal of cells for cell systems biology and pharmacology Ye Fang, A. Ferrie, +6 authors R. Verkleeren Published 2007 Biology … greater boston legal services phone number