WebThe NeutrAvidin resin was provided as a 6% cross-linked beaded agarose matrix in 50% aqueous slurry with a binding capacity of approximately 50 nmol of biotinylated antibody/mL of immobilized NeutrAvidin protein. For each immobilization assay, approximately 200 pmol of each GFPuv variant was incubated with 100 lL of immobilized NeutrAvidin protein. Web21 de jun. de 2024 · The resin used for capturing the biotin-tagged proteins was High Capacity Neutravidin Agarose Resin (Thermo Scientific). The resin is composed of Neutravidin linked to CL-6B Sepharose, supplied as 50% slurry. The specifications of the matrix are 6% cross-linked spherical agarose with a particle size of 45-165 μm.
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WebIt is worth mentioning that the binding capacity of the High Capacity NeutrAvidin Agarose Resin is ~8 mg/ml as per manufacturer's specifications, meaning that AviTag-PCNA was coupled to the resin ... WebSuperior performance – High Capacity Resin has higher binding capacity than best alternative supplier streptavidin products, allowing use of small amounts of resin for … howling mine tcg
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WebThermo Scientific High Capacity NeutrAvidin Agarose is a beaded agarose resin of immobilized NeutrAvidin Protein, a modified form of avidin with exceptional biotin-binding … Web26 de abr. de 2024 · Cells were then lysed and incubated with high-capacity NeutrAvidin-agarose resin (Thermo Fisher Scientific, 29204). After three washes with lysis buffer, bound proteins were eluted in SDS sample buffer and separated using 10% SDS PAGE electrophoresis; they were then transferred to PVDF membranes. Web10 de jan. de 2024 · Superior performance – High Capacity Resin has higher binding capacity than best alternative supplier streptavidin products, allowing use of small amounts of resin for experiments; Item-Specific Features: Agarose Resins are crosslinked 6% beaded agarose; UltraLink Resins are beaded acrylamide support (UltraLink Biosupport) howling mine revised mtg