Ime intron plant
WitrynaIME; however, a large internal deletion of the first intron of the maize Adh gene reduces splicing and con sequently IME (Luehrsen and Walbot 1994b). A point mutation at the … Witryna26 lip 2024 · Sexuality and apomixis are interconnected plant reproductive routes possibly behaving as polyphenic traits under the influence of the environment. In the subtropical grass Paspalum notatum, one of the controllers of apospory, a main component of gametophytic apomixis reproduction, is TRIMETHYLGUANOSINE …
Ime intron plant
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WitrynaIntrons of plant tubulin genes are important regulatory elements for supporting gene expression. Introns can either increase the level of expression through intron-mediated enhancement (IME), or they can change the actual site of gene expression. The molecular basis of both IME and intron dependent spatial expression (IDSE) remains … Witryna22 paź 2024 · Plant biotechnology traits provide a means to increase crop yields, manage weeds and pests, and sustainably contribute to addressing the needs of a growing population. One of the key challenges in developing new traits for plant biotechnology is the availability of expression elements for efficacious and predictable …
Since it has been shown that specifically the first intron bears the capacity to influence expression of the gene it is part of, the set of Arabidopsis introns was split into two sets, one with only the first introns, i.e. the 5′-most, of each gene, and another for all remaining introns, termed “other introns”. … Zobacz więcej For identifying candidate intron motifs associated with IME, a k-mer-based strategy similar to IMEter was applied, with additionally utilizing conservation and relative position in introns as informative criteria, similarly … Zobacz więcej The set of hexamers contained sequence-related hexamer sequences, which may be equivalent in function or part of a larger consensus motif, … Zobacz więcej To further evaluate the newly identified motifs, they were compared to IMEter, the most commonly used tool for identifying potentially IME … Zobacz więcej WitrynaIntrons of plant tubulin genes are useful molecular tools to study IME (Intron Mediated Enhancement of gene expression) and to define plant genetic and evolutionary relationships through ILP (Intron Length Polymorphism). Here we show that the intron present within the 5′UTR sequence of some rice β-tubulin genes can sustain IME in …
Witryna28 kwi 2008 · However, the mechanism underlying intron-mediated enhancement (IME) in plants is largely unknown. Rose et al. (pages 543–551) adopted a bioinformatics …
WitrynaIntron-mediated enhancement (IME) is the ability of an intron sequence to enhance the expression of a gene containing that intron. ... In Arabidopsis and other plant species, the IMEter has been developed to calculate the likelihood that an intron sequence will enhance gene expression.
Witryna3 kwi 2024 · Previously generated transgenic TRP1:GUS plants with the first intron of UBQ10 at three additional locations , designated herein as positions 0, −1, and −2, … floor method pythonWitryna12 mar 2024 · ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription … great places to eat in waco txWitryna24 cze 2024 · Consistent with plant and mammalian studies, we determined a single, natural or synthetic, 5′-intron is sufficient for the full IME effect conferred by three … great places to eat lunchWitryna1 lut 2000 · In plants, the inclusion of one or more introns in a gene construct usually leads to increased accumulation of mRNA and protein relative to similar fusions that … great places to eat in virginia beachWitrynaHere we show that the intron present within the 5′UTR sequence of some rice β-tubulin genes can sustain IME in rice transgenic plants and that degenerated oligonucleotide mixtures designed to amplify the first and the second intron present within the coding sequence of plant β-tubulin genes can successfully detect ILPs among different bean ... great places to eat lunch in pittsburghWitryna31 maj 2024 · Contrary to IME observed in yeast cells [61,62], splicing is not required for plant-stimulating introns [79,80] The UBQ10 first intron not only increases mRNA accumulation, but also affects tissue specificity when the reporter gene containing the UBQ10 first intron is under the control of different promoters , suggesting that UBQ10 … floor method in c#WitrynaAn intron is any nucleotide sequence within a gene that is not expressed or operative in the final RNA product. The word intron is derived from the term intragenic region, i.e., a region inside a gene. The term intron refers to both the DNA sequence within a gene and the corresponding RNA sequence in RNA transcripts. The non-intron sequences that … great places to eat in tampa fl